Learn what you can do to pinpoint the correct date of mating.
The early readings are: .1 - .2 .3
Start to draw blood on the 7th day.
They can go back down to .2 or even .1 – this depends on the time of day the blood was drawn
When the LH surge goes over 1.0 to 1.9 – you breed in 5 to 6 days
If the 5th day seems a little early – you breed on the 6th and 7th day – then skip a day and breed again.
If you draw blood and the level is .2 or .3 – then wait 3 days and draw blood again.
If you draw blood and the level is .7 or .8 – then you do it every other day
Once you start to see the level at:
2.0 to 2.9 ng/ml = breed in 4 to 5 days
3.0 to 3.9 ng/ml = breed in 3 to 4 days
4.0 to 8 .0 ng/ml = breed today or tomorrow - this is where the LH Surge Occurs.
Over 8.0 ng/ml with no white cells – then the bitch has ovulated
When you have bitches that do not swell up or have a lot of discharge – good idea to have their thyroid level checked.
My personal experience with progesterone testing has been good. Usually I test the female at 7 days for the first time. Then again two days later. Depending on the results, I may test again two to three days after that. The best tip is that when the progesterone level jumps over 1.0 ng/ml, the female will usually be ready to breed in 4-5 days. If the progesterone level is 5.0ng/ml
or over but less than 10.0ng/ml, breeding should be done now.
Breeding the female depends on her receptiveness to the male and when the mating is good, I usually try to get another mating the day after the day following the initial mating. The sperm stays viable for 24-48 hours so you will have covered 4-5 days of estrus with the two matings. It is not the number of times you breed the dogs but the day on which you do it. Remember, if you breed your female for an entire week, you will not be able to pinpoint the day of whelping very well.
If you have difficulty in determining your female's ovulation window, there are other tests that you can do to pinpoint the correct day. Ask your veterinarian or consult with a veterinarian specializing in fertility.